stem cell surface markers Search Results


90
ZenBio stem cell surface markers
Adipose <t>stem</t> cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean <t>cell</t> number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (**** p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell <t>surface</t> expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines
Stem Cell Surface Markers, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stem cell surface markers - by Bioz Stars, 2026-03
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90
Pharmagen gmbh bone marrow stem cell surface markers
Adipose <t>stem</t> cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean <t>cell</t> number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (**** p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell <t>surface</t> expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines
Bone Marrow Stem Cell Surface Markers, supplied by Pharmagen gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
bone marrow stem cell surface markers - by Bioz Stars, 2026-03
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90
OncoMed Inc discovery of surface markers for the differential purification of cancer stem cell populations from human malignancies
Adipose <t>stem</t> cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean <t>cell</t> number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (**** p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell <t>surface</t> expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines
Discovery Of Surface Markers For The Differential Purification Of Cancer Stem Cell Populations From Human Malignancies, supplied by OncoMed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/discovery of surface markers for the differential purification of cancer stem cell populations from human malignancies/product/OncoMed Inc
Average 90 stars, based on 1 article reviews
discovery of surface markers for the differential purification of cancer stem cell populations from human malignancies - by Bioz Stars, 2026-03
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90
Quanticel Pharmaceuticals discovery of surface markers for the differential purification of cancer stem cell populations from human malignancies
Adipose <t>stem</t> cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean <t>cell</t> number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (**** p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell <t>surface</t> expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines
Discovery Of Surface Markers For The Differential Purification Of Cancer Stem Cell Populations From Human Malignancies, supplied by Quanticel Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/discovery of surface markers for the differential purification of cancer stem cell populations from human malignancies/product/Quanticel Pharmaceuticals
Average 90 stars, based on 1 article reviews
discovery of surface markers for the differential purification of cancer stem cell populations from human malignancies - by Bioz Stars, 2026-03
90/100 stars
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Adipose stem cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean cell number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (**** p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell surface expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines

Journal: Breast Cancer Research and Treatment

Article Title: Adipose stem cell crosstalk with chemo-residual breast cancer cells: implications for tumor recurrence

doi: 10.1007/s10549-018-05103-w

Figure Lengend Snippet: Adipose stem cells (ASCs) Migrate toward Conditioned Media (CM) from Chemo-Residual TNBC Cells. A CM was prepared by growing chemo-residual SUM159 TNBC cells in serum-free media for 48 h. The ability of primary human ASCs (Zenbio) to migrate toward this CM (Chemo-residual CM), FBS (+ Control) or serum-free media (− Control) was evaluated in a 15 h transwell assay. Total number of migrated cells from 5 representative fields (100 × magnification) was determined for each well, and mean cell number from triplicate wells (± SD) was calculated. Significance was determined by two tailed t test (**** p < 0.0001). Similar results were obtained in at least three independent trials. B Representative fields (100 ×) showing migration of ASCs towards chemo-residual TNBC CM in a 15 h transwell assay. C CXCR4 expression was assessed by incubating ASCs with CXCR4 antibody (blue line) or control IgG (red line), followed by FITC-conjugated secondary antibody. CXCR4 cell surface expression was determined by flow cytometry. D RNA was isolated from untreated and chemo-residual SUM159 cells and subjected to SDF-1 alpha and GAPDH real-time PCR. SDF-1 alpha gene expression relative to GAPDH is presented for both cell lines

Article Snippet: These cells were shown by Zenbio to express stem cell surface markers, as well as the ability to differentiate into adipocytes, chondrocytes, and osteocytes.

Techniques: Control, Transwell Assay, Two Tailed Test, Migration, Expressing, Flow Cytometry, Isolation, Real-time Polymerase Chain Reaction, Gene Expression